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ptwin1 vector  (New England Biolabs)


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    Structured Review

    New England Biolabs ptwin1 vector
    Ptwin1 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptwin1+vector/pTWIN1+Vector+DNA/pmc12032424-132-25-27
    Average 93 stars, based on 218 article reviews
    ptwin1 vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Subcloning:

    Article Title: Adenosine Triphosphate Stimulates Aquifex aeolicus MutL Endonuclease Activity
    Article Snippet: .. The forward and reverse primers incorporated NdeI and SapI restriction sites, respectively, for subcloning into the pTWIN1 vector (New England Biolabs, Inc.). .. The PCR product was digested with NdeI and SapI restriction endonucleases (New England Biolabs, Inc) and subcloned into the same sites in pTWIN1 to create a direct fusion with the Mxe GyrA Intein-tag.

    Plasmid Preparation:

    Article Title: Phosphorylation and O-GlcNAcylation at the same α-synuclein site generate distinct fibril structures
    Article Snippet: .. To obtain segment X, α-syn 1-84 was introduced into pTWIN1 vector (NEB) containing intein and chitin binding domain. ..

    Article Title: Semi-synthesis of TDP-43 reveals the effects of phosphorylation in N-terminal domain on self-association
    Article Snippet: .. The plasmid pTDP 50-414 , where the TDP 50-414 gene with CBD and Ssp DnaB intein (Intein) genes at its N-terminus is cloned into the pTWIN1 vector (New England Biolabs Inc., Ipswich, MA), was designed (Fig. , plasmid map drawing was prepared with SnapGene [GSLBiotech LLC, Boston, MA] [ www.snapgene.com ]) and purchased from GenScript (Piscataway, NJ). .. T7 Express Competent E. coli host strain cells (New England Biolabs Inc., Ipswich, MA) were transformed with the plasmid and grown on Luria-Bertani (LB) agar medium supplemented with 100 μg/mL ampicillin.

    Article Title: Applying selenocysteine-mediated expressed protein ligation to prepare the membrane enzyme selenoprotein S
    Article Snippet: .. MBP-TEV-selenos 1-187 and MBP-TEV-selenos 52-187 are cloned into the pTWIN1 vector (NEB) using NdeI and SapI cloning sites. .. Reagents for molecular cloning 1 The expression vector pTWIN1 (NEB).

    Article Title: Semi-synthesis of TDP-43 reveals the effects of phosphorylation in N-terminal domain on self-association.
    Article Snippet: .. TheplasmidpTDP50-414,where theTDP50-414 genewithCBDand SspDnaB intein (Intein) genes at its N-terminus is cloned into the pTWIN1 vector (New England Biolabs Inc., Ipswich, MA), was designed (Fig. S19, plasmid map drawing was prepared with SnapGene [GSLBiotech LLC, Boston, MA] [www.snapgene.com]) and purchased from GenScript (Piscataway, NJ). .. T7 Express Competent E. coli host strain cells (New England Biolabs Inc., Ipswich,MA)were transformedwith the plasmid and grown on LuriaBertani (LB) agar medium supplemented with 100 μg/mL ampicillin.

    Binding Assay:

    Article Title: Phosphorylation and O-GlcNAcylation at the same α-synuclein site generate distinct fibril structures
    Article Snippet: .. To obtain segment X, α-syn 1-84 was introduced into pTWIN1 vector (NEB) containing intein and chitin binding domain. ..

    Clone Assay:

    Article Title: Semi-synthesis of TDP-43 reveals the effects of phosphorylation in N-terminal domain on self-association
    Article Snippet: .. The plasmid pTDP 50-414 , where the TDP 50-414 gene with CBD and Ssp DnaB intein (Intein) genes at its N-terminus is cloned into the pTWIN1 vector (New England Biolabs Inc., Ipswich, MA), was designed (Fig. , plasmid map drawing was prepared with SnapGene [GSLBiotech LLC, Boston, MA] [ www.snapgene.com ]) and purchased from GenScript (Piscataway, NJ). .. T7 Express Competent E. coli host strain cells (New England Biolabs Inc., Ipswich, MA) were transformed with the plasmid and grown on Luria-Bertani (LB) agar medium supplemented with 100 μg/mL ampicillin.

    Article Title: Applying selenocysteine-mediated expressed protein ligation to prepare the membrane enzyme selenoprotein S
    Article Snippet: .. MBP-TEV-selenos 1-187 and MBP-TEV-selenos 52-187 are cloned into the pTWIN1 vector (NEB) using NdeI and SapI cloning sites. .. Reagents for molecular cloning 1 The expression vector pTWIN1 (NEB).

    Article Title: Semi-synthesis of TDP-43 reveals the effects of phosphorylation in N-terminal domain on self-association.
    Article Snippet: .. TheplasmidpTDP50-414,where theTDP50-414 genewithCBDand SspDnaB intein (Intein) genes at its N-terminus is cloned into the pTWIN1 vector (New England Biolabs Inc., Ipswich, MA), was designed (Fig. S19, plasmid map drawing was prepared with SnapGene [GSLBiotech LLC, Boston, MA] [www.snapgene.com]) and purchased from GenScript (Piscataway, NJ). .. T7 Express Competent E. coli host strain cells (New England Biolabs Inc., Ipswich,MA)were transformedwith the plasmid and grown on LuriaBertani (LB) agar medium supplemented with 100 μg/mL ampicillin.

    Article Title: Different phosphorylation and farnesylation patterns tune Rnd3–14-3-3 interaction in distinct mechanisms
    Article Snippet: .. In order to generate 14-3-3ζ without additional tag, the 14-3-3ζ gene was cloned into the pTWIN1 Vector (New England Biolabs Inc.) before the gene of intein-CBD. .. For higher cutting efficiency, we inserted glycine at the end of 14-3-3ζ (The last amino acid on 14-3-3ζ is asparagine, which is hardly cleaved when it is adjacent to the intein3).The designed primers are showed in table 2,The constructed plasmid was transformed into BL21 (DE3) competent E. coli (New England Biolabs Inc.) and expressed.

    Polymerase Chain Reaction:

    Article Title: Recycling of hyoscyamine 6β-hydroxylase for the in vitro production of anisodamine and scopolamine.
    Article Snippet: The tropane alkaloids hyoscyamine, anisodamine, and scopolamine are extensively used medicines.. In particular, scopolamine has the greatest value in the market.. Hence, strategies to enhance its production have been explored as an alternative to traditional field-plant cultivation.

    Protease Inhibitor:

    Article Title: Recycling of hyoscyamine 6β-hydroxylase for the in vitro production of anisodamine and scopolamine.
    Article Snippet: The tropane alkaloids hyoscyamine, anisodamine, and scopolamine are extensively used medicines.. In particular, scopolamine has the greatest value in the market.. Hence, strategies to enhance its production have been explored as an alternative to traditional field-plant cultivation.



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